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Author: Admin | 2025-04-28
Recorded at λ1 and λ2. The percentage recovery was calculated by measuring the absorbance of both drug at their absorbance maxima and fitting these values into simultaneous equation. Each response was average of three determinations.Limit of detection and Limit of quantitationThe limit of detection (LOD) and the limit of quantitation (LOQ) of the drug were derived by calculating the signal-to-noise ratio (S/N, i.e., 3.3 for LOD and 10 for LOQ) using the following equations designated by International Conference on Harmonization (ICH) guidelines.LOD = 3.3 × σ/SLOQ = 10 × σ/SWhere, σ = the standard deviation of the response and S = slope of the calibration curve.Analysis of tablet dosage formTake 10 tablets and weighed. Find out average weight. Take tablet powder equivalent to 50 mg of SIL and 30 mg of DPX was transferred in 100 ml volumetric flask, dissolved and diluted up to mark with methanol. The solution was sonicated for 15minutes. Filter the solution through Whatman filter paper no.42 and discard first few drops of filtrate. Pipette out 0.2 ml of the above solution in 10ml volumetric flask and diluted to mark with methanol. Absorbance of the resulting solution was measured at 291.0 nm and 230.0 nm against methanol, relative concentration of two drugs in the sample was calculated using above equation (1) and (2).RESULT AND DISCUSSIONIn simultaneous equation method, the primary requirement for developing a method for analysis is that the entire spectra should follow the Beer’s law at all the wavelength, which was fulfilled in case
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